Characterization of Physical Gap Sizes at Human Telomeres

Functional characterization of the TERRA transcriptome at damaged telomeres.

Telomere deprotection occurs during tumorigenesis and aging upon telomere shortening or loss of the telomeric shelterin component TRF2. Deprotected telomeres undergo changes in chromatin structure and elicit a DNA damage response (DDR) that leads to cellular senescence. The telomeric long noncoding RNA TERRA has been implicated in modulating the structure and processing of deprotected telomeres...

Characterization of short tandem repeats from thirty-one human telomeres.

Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiati...

Characterization of Human Glucose - 6 - Phosphate Isomerase of Different Sizes

Telomeres at a glance.

The linearity of chromosomes creates two major problems for eukaryotic cells: the end-replication problem and the endprotection problem. The end-replication problem stems from the inherent inability of the replication machinery to fully duplicate linear templates. The endprotection problem refers to the propensity of linear chromosome ends to be recognized as DNA double-strand breaks (DSBs). Bo...

TERRA, hnRNP A1, and DNA-PKcs Interactions at Human Telomeres

Maintenance of telomeres, repetitive elements at eukaryotic chromosomal termini, and the end-capping structure and function they provide, are imperative for preserving genome integrity and stability. The discovery that telomeres are transcribed into telomere repeat containing RNA (TERRA) has revolutionized our view of this repetitive, rather unappreciated region of the genome. We have previousl...